https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/Recent content in Genome engineering on BioeconHugoen-UShttps://en.bioecon.ru/docs/synbio-enabling/genome-engineering/xenobiology-expanded-genetic-alphabet/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/xenobiology-expanded-genetic-alphabet/Building genetics that the rest of the biosphere cannot read — and the several distinct chemistries that get there.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/base-editing/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/base-editing/Chemistry on a single displaced DNA strand — no break, no template, and a window that edits more than you asked for.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/biosafety-lab-biosecurity/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/biosafety-lab-biosecurity/Containment is airflow, pressure and filtration doing arithmetic — and at the highest level it fails on procedure, not on engineering.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/crispr-screening-as-a-service/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/crispr-screening-as-a-service/A pooled screen is a statistical experiment whose answer is decided before any biology happens — by coverage, infection rate and the choice of selection.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/dna-synthesis-biosecurity-screening/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/dna-synthesis-biosecurity-screening/Screening an order against a list of dangerous sequences works exactly as well as sequence similarity does — and there are three ways around it.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/ecological-engineering-gene-drives/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/ecological-engineering-gene-drives/Inheritance biased above one half — and the resistance allele the mechanism generates every time it fails.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/epigenome-editing/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/epigenome-editing/Changing whether a gene is read without changing what it says — and the one question that decides whether the change lasts.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/genome-editing/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/genome-editing/The nuclease only makes a break. What the edit becomes is decided by the cell’s repair machinery.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/intelligent-genetic-switches-kill-switches/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/intelligent-genetic-switches-kill-switches/Containment is not a feature but a rate — and the rate is set by how many mutants the population can afford to make.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/minimal-genomes-synthetic-cells/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/minimal-genomes-synthetic-cells/A bacterium reduced to 473 genes still contains scores whose function nobody knows — and that is the result, not a footnote.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/prime-editing/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/prime-editing/A reverse transcriptase writing new DNA from a template carried by the guide itself — and why the guide design is the whole engineering problem.https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/synthetic-biology/Mon, 01 Jan 0001 00:00:00 +0000https://en.bioecon.ru/docs/synbio-enabling/genome-engineering/synthetic-biology/The attempt to make biology composable — and the four reasons a circuit that worked on its own stops working inside a cell.