technology

Size-exclusion chromatography (SEC)

Separation by molecular size as a sample passes through a porous stationary phase, used to quantify protein aggregates and monomer purity in biotherapeutics.

Where it's used

HPLC/UPLC instruments for biopharma
Bench-top liquid chromatography systems — HPLC and ultra-high-performance UPLC platforms — that separate and quantify proteins, peptides, small molecules and impurities ahead of UV, fluorescence or mass-spectrometry detection, the workhorse instrument for biopharma purity testing, release assays and formulation characterization.
analytics-pat 7 min