technology

Trypan blue exclusion

Viability assay in which dead cells with compromised membranes take up trypan blue dye while live cells exclude it, distinguishing live from dead cells under bright-field imaging.

Where it's used

Cell counters and viability systems
Bench-top instruments that automate cell counting and viability assessment — image-based dye-exclusion counters, fluorescence-based analyzers and closed-cassette systems — replacing manual hemocytometer counting for daily cell-culture QC, bioreactor process monitoring and cell-therapy/cell-line release testing.
analytics-pat 7 min