Plant tissue culture and micropropagation
In vitro multiplication of plants from small tissue pieces on sterile nutrient media, giving clonal, disease-free planting material.
1 · What it gives
Micropropagation is widely used for banana, potato seed, ornamentals, orchids and some tree crops, and for virus-free stock. It needs a sterile laboratory, trained staff and phytosanitary certification for trade. The portal covers animal-cell culture equipment separately; this page is about plants.
- Explant selection and sterilisation: Take shoot tips or other tissue from a healthy mother plant and surface-sterilise it.
- Initiation and multiplication: Grow explants on media with plant hormones so they form multiple shoots; subculture repeatedly.
- Rooting: Move shoots to rooting medium to develop roots.
- Acclimatisation: Transfer plantlets to greenhouse conditions with gradual humidity reduction before field sale.
Draft from established general knowledge; not source-checked (verified: false, confidence: low).
2 · What it replaces 6 · matched by industry
| D3E2 | Peat-free and peat-reduced growing media (coir, wood fibre, composted bark, green-waste compost, sphagnum farming) instead of extracted peat Replaces: Peat (sphagnum moss peat) as the main constituent of potting soils and professional horticultural substrates | AGROCHEM |
| D1E2 | Disease-resistant livestock genetics — genomic selection, adapted breeds, gene-edited PRRS-resistant pigs — instead of routine antibiotics, acaricides and repeated mass treatment Replaces: Metaphylactic antibiotics used against secondary infections after PRRS and other viral diseases; frequent acaricide dipping of … | BREED |
| D2E– | Grafting onto resistant rootstocks, biological/anaerobic soil disinfestation and biofumigation, and soilless (substrate and hydroponic) culture instead of methyl bromide and chemical soil fumigants Replaces: Methyl bromide (phased out under the Montreal Protocol, remaining critical/quarantine uses); chemical fumigants used in its place — metam … | SERV |
| D3E– | Augmentative biological control in greenhouse vegetables instead of insecticide and acaricide programmes Replaces: Calendar sprays of insecticides and acaricides (organophosphates, neonicotinoids, pyrethroids, abamectin) against whitefly, thrips, aphids, … | MACRO |
| D2E– | Bumblebee pollination instead of hormone sprays and manual vibration in greenhouse tomatoes Replaces: Fruit-setting hormone sprays (synthetic auxins such as 4-CPA / tomatotone) and labour-intensive manual flower vibration in greenhouse … | MACRO |
| D2E– | Disease-resistant crop varieties (marker-assisted breeding, stacked resistance genes, cisgenesis) instead of routine fungicide spray programmes Replaces: Season-long protective fungicide programmes on susceptible varieties — e.g. 10–20 sprays of mancozeb/chlorothalonil/metalaxyl against potato … | SEED |
3 · How to implement 4 steps
- 1Explant selection and sterilisationTake shoot tips or other tissue from a healthy mother plant and surface-sterilise it.
- 2Initiation and multiplicationGrow explants on media with plant hormones so they form multiple shoots; subculture repeatedly.
- 3RootingMove shoots to rooting medium to develop roots.
- 4AcclimatisationTransfer plantlets to greenhouse conditions with gradual humidity reduction before field sale.
4 · Who delivers
Matched suppliers (matched automatically)
| Hektaş (BIST: HEKTS) | Turkey | Producer | active |
| Wakefield BioChar | United States | Producer | active |
| Biobest | Belgium | Services / consulting | active |
| GDM (Don Mario) | Argentina | Producer | active |
| INIA Chile | Chile | Research / RTO | active |
| International Center for Biosaline Agriculture (ICBA) | United Arab Emirates | Research / RTO | active |
| Kaveri Seed Company (NSE) | India | Services / consulting | active |
| Multiplex Group | India | Research / RTO | active |
| Rasi Seeds | India | Producer | active |
| Rijk Zwaan | Netherlands | Distributor | active |
| Singrow | Singapore | Producer | active |
| Takii & Sakata Seed | Japan | Research / RTO | active |
Leading companies and institutes (from the brief)
5 · Rules & route to market
about 1-2 years incl. registration trials
Information, not legal advice — confirm the procedure for your product. (2026-10-06)
United States
Policy documents mention tissue culture for Sri Lanka, Tanzania, Uganda and Asia-Pacific.
European Union
Contamination losses, somaclonal variation and the cost of skilled labour are the usual risks to check.
Sources:
- none matched yet
China
No companies are linked yet; the portal’s supplier matching adds them under the related industries.
6 · Money: tenders & grants
| Grant | Strengthening plant health: addressing emerging plant pest risks European Commission · EU | Apr 8, 2027 174 days |
7 · Tech stack & innovations
Where this appears 6 sources
Appears in 6 distinct sources across 6 regions, 2008–2026.
- policy documents 6
Regions: Asia-Pacific · Pakistan · Paraguay · Sri Lanka · Tanzania · Uganda
- 2021: 0
- 2022: 0
- 2023: 0
- 2024: 0
- 2025: 11
- 2026: 11
- 2027: 0
Matched themes 3
Micropropagación Plant Tissue Culture Tissue Culture
Themes from forum programmes, policy documents and reports are mapped to technologies automatically and then reviewed; counts are distinct sources, not mentions, and are not a ranking. data sources and method → · Trending, not yet covered →
Sources 1 · 0 independent · 1 producer sites
Producers' own sites 1
- www-pub.iaea.org producer checked 2026-11-05