Soil health certification & assessment
Metagenomic sequencing platforms that assess soil microbiome composition and function, generating standardized soil-health passports used to verify carbon-credit claims, guide biological input decisions and satisfy emerging government soil-health certification programs.
1 · What it gives
Soil health certification and assessment uses high-throughput metagenomic DNA sequencing of soil samples to generate a standardized, verifiable “passport” of microbiome composition and function — replacing subjective visual soil assessment with data that can support carbon-credit verification, biological-input recommendations and government soil-health compliance programs. Biome Makers, dual-headquartered in the US and Spain, launched BeCrop 4.0 in March 2026, a major upgrade to its AI-powered soil-intelligence platform improving DNA taxonomic assignment precision by over 30% and identifying more than 44,550 fungal species; the company’s underlying BeCrop platform was originally developed with EU Horizon 2020 funding for early biological-threat detection in agriculture. Trace Genomics, a Bay Area soil-metagenomics pioneer, was acquired by Canadian company Miraterra in July 2025 to combine molecular soil biology with Raman spectroscopy into an integrated “soil-to-table intelligence” platform. Pattern Ag continues expanding its predictive soil-pathogen and nematode testing service, having grown into cotton-specific predictive analytics for the US Delta region. Loam Bio, an Australian agricultural microbial company, markets CarbonBuilder, a fungal furrow treatment applied to wheat, barley and canola that uses root-associated fungi to sequester CO2 into stable soil carbon, with university trials demonstrating soil carbon gains as high as 9.4%. In China, the Institute of Soil Science, Chinese Academy of Sciences (based in Nanjing, tracing its origins to a 1930 soil research office), hosts the National Key Laboratory of Soil and Sustainable Agriculture and continues to publish foundational soil-health research, including 2025-2026 work on soil microbiome-function relationships for sustainable agriculture and ecosystem resilience.
The key directions of soil health certification and assessment are:
- Metagenomic soil health passporting: next-generation sequencing (16S/ITS marker genes) of soil DNA to generate a standardized, comparable digital record of microbial community composition.
- Predictive pathogen and pest risk scoring: using sequencing data to forecast disease and pest pressure several months ahead of visible symptoms, informing preventive input decisions.
- Soil carbon sequestration verification: analyzing fungal-to-bacterial biomass ratios and melanized endophytic fungi content to verify that carbon has been stably fixed in soil, supporting carbon-credit issuance.
- Government soil-health standardization: national programs (such as USDA soil-health initiatives) that fund and standardize biological soil-activity assessment methods across farms.
Sectoral value chain#
[GPS-gridded soil sampling] ──> [DNA extraction] ──> [16S/ITS library prep & NGS sequencing]
│
(Bioinformatic taxonomic & functional analysis)
│
[Soil regeneration & carbon credit issuance] <──── [Agronomic consulting & prescription] <───┘
Value chain levels#
| Level | Description | Key inputs/outputs |
|---|---|---|
| Soil sampling | Systematic 0-20 cm soil core collection on a precision GPS grid with geographic tagging. | In: Automated samplers, GPS terminals, sterile bags. Out: Georeferenced, standardized soil samples. |
| DNA extraction | Disrupting soil microaggregates, lysing bacterial and fungal cells, and isolating total DNA. | In: Soil sample, DNA extraction kits, centrifuges. Out: High-quality purified genomic DNA solution. |
| Library prep and NGS | Preparing DNA libraries (16S/ITS marker amplification) and running sequencing on Illumina/Oxford Nanopore platforms. | In: Purified DNA, primers, enzymes, sequencers. Out: Raw genetic read files (FASTQ). |
| Bioinformatics | Noise filtering, read assembly, taxonomic classification and AI-driven functional network analysis. | In: FASTQ read files, reference databases (SILVA, UNITE). Out: Interactive soil-health functional passport. |
| Agronomic consulting | Translating bioinformatic data into practical agronomic decisions and biological-input dosing. | In: Soil passport, target-crop requirements, biological-input databases. Out: Digital biological-regeneration prescription map. |
| Soil regeneration | Applying recommended microbial consortia, cover-crop seeding, no-till adoption and microbiome-recovery monitoring. | In: Microbial products, cover-crop seed, no-till seeders. Out: Restored, suppressive soil with verified carbon potential. |
Cross-cutting technologies of the sector:
- Soil metagenomic sequencing: high-throughput NGS of soil DNA using 16S (bacterial) and ITS (fungal) marker genes to census the soil microbial community and decode its functional gene networks.
- Fungal-to-bacterial biomass ratio analysis: measuring the relative abundance of fungal versus bacterial biomass as a proxy indicator of soil structural stability, organic-matter turnover and carbon-sequestration capacity.
- Endophyte carbon verification: confirming the presence and activity of melanized endophytic fungi that convert plant-derived carbon into stable soil aggregates, a key evidence requirement for soil-carbon-credit certification.
2 · What it replaces 4 · matched by industry
3 · How to implement 6 steps
- 1GPS-gridded soil core samplingField technicians collect standardized 0-20 cm soil cores across a precision GPS grid, using automated samplers and sterile collection bags to prevent cross-contamination between sampling points.
- 2DNA extraction and purificationPurification & downstreamExtractionSoil microaggregates are mechanically disrupted, bacterial and fungal cell walls are lysed with specialized extraction kits, and total genomic DNA is purified to a concentration and quality suitable for downstream sequencing.Who can do this step matched automaticallyEsco Aster Singapore✓ ISO 9001 5,000 L industrial scale“Adherent cell culture and downstream processing: commercialized Tide Motion bioreactor platform (MiniTide to TideXcell) with closed automated cell harvesting and single-use …”HLB Genex (ex-GenoFocus) South Koreaindustrial scale“With fungal and bacterial secretion systems, we custom produce the evolved enzyme products in large-scale fermentation and downstream processes.”
- 3Library preparation and NGS sequencingTesting & analyticsFormulation & blendingPurified DNA undergoes 16S (bacterial) and ITS (fungal) marker-gene amplification to build sequencing libraries, which are run on Illumina or Oxford Nanopore platforms to generate raw genetic read data.Who can do this step matched automaticallyEsco Aster Singapore✓ ISO 9001 5,000 L industrial scale“Esco Aster (Singapore) is a cGMP contract research, development and manufacturing organization (CRDMO/CDMO) and bioinnovation venture accelerator that helps clients and …”500,000 tons pilot scale“It refines sugar (including refined white sugar, golden brown sugar, fine granulated sugar and invert sugar syrup), produces bio-based chemicals/biotech outputs (alcohol, …”Rossari Biotech (NSE) India✓ ISO 9001 industrial scale“The company also announced a new greenfield blending facility in Thailand to boost Southeast Asia operations and smarter capacity utilization across its HPPC, TSC, and AHN …”✓ ISO 9001 500 m3“This is a satellite blending, mixing and formulation facility, located at Vashind near Shahapur and dedicated to the company’s Animal Nutrition business.”
- 4Bioinformatic taxonomic and functional analysisTesting & analyticsData, software & automationRaw sequencing reads are filtered for noise, assembled and classified against reference databases (SILVA for bacteria, UNITE for fungi), with AI-driven analysis mapping functional gene networks into an interactive digital soil-health passport.Who can do this step matched automaticallyEsco Aster Singapore✓ ISO 9001 5,000 L industrial scale“Esco Aster (Singapore) is a cGMP contract research, development and manufacturing organization (CRDMO/CDMO) and bioinnovation venture accelerator that helps clients and …”163,050 tonnes“As the fundamental component of 5G deployment, optic fibre is critical to making Hong Kong a smart city and laying the groundwork for IoT.”
- 5Agronomic prescription and carbon scoringThe soil-health passport data is translated into practical recommendations for biological-input dosing, cover-crop selection and tillage practice, alongside a carbon-sequestration score based on fungal-to-bacterial ratios and endophyte content.
- 6Regeneration and carbon-credit verificationTesting & analyticsFarmers apply the recommended microbial consortia and regenerative practices, with follow-up sampling confirming microbiome recovery and providing the metagenomic evidence required to verify and issue soil-carbon credits.Who can do this step matched automaticallyEsco Aster Singapore✓ ISO 9001 5,000 L industrial scale“Esco Aster (Singapore) is a cGMP contract research, development and manufacturing organization (CRDMO/CDMO) and bioinnovation venture accelerator that helps clients and …”
4 · Who delivers
Matched suppliers (matched automatically)
| Wakefield BioChar | United States | Producer | active |
In the services and supply catalogue 2 · paid service
Analytical & testing services · Regulatory & certification
Leading companies and institutes (from the brief)
| Company / Institute | Country | Key products / platforms | Tech features | Status 2026 |
|---|---|---|---|---|
| Biome Makers | 🇺🇸 USA | BeCrop 4.0 soil-intelligence platform | 44,550+ fungal species identified, EU Horizon origins | operating |
| Trace Genomics | 🇺🇸 USA | Soil-to-table intelligence (with Miraterra) | Metagenomics + Raman spectroscopy integration | commercial |
| Pattern Ag | 🇺🇸 USA | Predictive soil-pathogen/nematode testing | Cotton-specific predictive analytics (Delta region) | operating |
| Loam Bio | 🇦🇺 Australia | CarbonBuilder fungal furrow treatment | Endophyte-based soil carbon sequestration | operating |
| ISSCAS | 🇨🇳 China | National soil-health research infrastructure | Century-old soil science mandate, national field stations | research |
5 · Rules & route to market
about 1-2 years incl. registration trials
Information, not legal advice — confirm the procedure for your product. (2026-10-06)
United States
The United States hosts the world’s leading soil-metagenomics platforms, increasingly consolidating through acquisition and expanding into crop-specific predictive analytics.
Biome Makers’ BeCrop 4.0 upgrade, Trace Genomics’ acquisition by Miraterra, Pattern Ag’s cotton-specific expansion#
- Biome Makers: launched BeCrop 4.0 in March 2026, improving DNA taxonomic assignment precision by over 30% and identifying more than 44,550 fungal species for better detection of beneficial and pathogenic organisms.
- Trace Genomics: acquired by Canadian company Miraterra in July 2025, combining Trace’s molecular soil biology with Miraterra’s Raman spectroscopy into an integrated “soil-to-table intelligence” platform.
- Pattern Ag: continues expanding its predictive soil-pathogen and nematode testing service, having grown from row-crop pathogen detection into cotton-specific predictive analytics for the US Delta region.
European Union
The European Union funds soil-microbiome assessment technology directly through Horizon research programs and increasingly ties soil health verification to carbon-farming payment schemes.
Horizon Europe-funded BeCrop origins, EU carbon-farming certification demand, Loam Bio’s endophyte carbon verification#
- Horizon Europe BeCrop origins: Biome Makers’ underlying soil-microbiome platform was originally developed with EU Horizon 2020 funding for early biological-threat detection in agriculture, illustrating direct European public investment in soil-metagenomics infrastructure.
- Carbon-farming certification demand: EU carbon-removal certification schemes increasingly require metagenomic verification that sequestered carbon is stably fixed by beneficial fungal communities rather than simply added as organic matter subject to rapid mineralization.
- Loam Bio (Australia, EU-relevant model): markets CarbonBuilder, a fungal furrow treatment for wheat, barley and canola using root-associated fungi to sequester CO2 into stable soil carbon, with university trials demonstrating soil carbon gains as high as 9.4% — a verification and product model increasingly referenced by European carbon-farming certification schemes.
China
China channels soil health research through its national academic soil-science infrastructure, anchored by one of the world’s oldest dedicated soil research institutions.
ISSCAS’s century-old soil science mandate, national soil-health laboratory infrastructure, sustainable agriculture research focus#
- Institute of Soil Science, Chinese Academy of Sciences (ISSCAS): based in Nanjing and tracing its origins to a 1930 soil research office, hosts the National Key Laboratory of Soil and Sustainable Agriculture and the National Engineering Research Center for Soil Nutrient Management and Pollution Remediation.
- Research output: continues to publish foundational soil-health research, including 2025-2026 work on the relationship between soil microbiome function and ecosystem resilience for sustainable agriculture.
- National infrastructure: operates multiple national field observation and research stations across China (including sites in Henan, Jiangxi and Jiangsu provinces), forming the backbone of China’s long-term soil-monitoring network.
6 · Money: tenders & grants
| Grant | Health of ecosystems and wild species, predictions and impacts on human health, in the face of existing and … European Commission · EU | Sep 22, 2027 341 days |
| Grant | Innovative biotechnologies to restore soil health and improve agricultural competitiveness and resilience European Commission · EU | Apr 8, 2027 174 days |
| Grant | Microbiome for terrestrial livestock sustainability and health within a One Health approach European Commission · EU | Sep 23, 2027 342 days |
| Grant | Strengthening plant health: addressing emerging plant pest risks European Commission · EU | Apr 8, 2027 174 days |
| Grant | Advancing Global Health Bureau of Global Health Security and Diplomacy · United States | Feb 14, 2027 121 days |
7 · Tech stack & innovations
The soil health certification and assessment stack combines DNA sequencing with bioinformatic and carbon-verification analytics:
- Soil metagenomic sequencing:
- High-throughput next-generation sequencing of soil DNA using 16S bacterial and ITS fungal marker genes generates a comprehensive census of the soil microbial community, decoding functional gene networks that predict nutrient cycling and disease-suppression capacity.
- Fungal-to-bacterial biomass ratio analysis:
- Measuring the relative abundance of fungal versus bacterial biomass serves as a proxy indicator for soil structural stability and long-term carbon-sequestration capacity, since fungal-dominated soils typically retain organic carbon in more stable forms.
- Endophyte carbon verification:
- Confirming the presence and activity of melanized endophytic fungi that convert plant-derived carbon into stable soil aggregates provides the evidentiary basis required for soil-carbon-credit certification and payment.
Sources 5 · 0 independent · 5 producer sites
Producers' own sites 5
- Biome makers producer checked 2026-05-22 · link not kept
- Trace genomics producer checked 2026-06-15 · link not kept
- Pattern ag producer checked 2026-06-25 · link not kept
- Loam bio producer checked 2026-05-13 · link not kept
- Isscas producer checked 2026-07-05 · link not kept